(tune of American Pie)

(v1)

G        D       Em 
Way down on the Kenai land
Am                           C      
That's where there lives that willow man
Em                              D 
Who sougt know the story of the willow rose
G          D        Em
So this tale's about a dad and kids
Am                C
And DNA barcoding kits
           Em                        C             D
Trying to know the midges that make those roses grow
Em                     Am
Well in the Old World and the New
Em                     Am
There've been some names at least a few
C              G         Am
Sometimes with vague descriptions
C                     D
and in great need of revision
G              D         Em
But In Europe through morphometry
Am                 D
It seems there's a plurality
G           D       Em
of cecidomyiids in this tree
          C               D7    G
We're not done with those cecid flies.


(chorus)
   G   C           G      D
So my, my how many cecidomyiids
               G            C           G          D  
Seems they're galling every Salix growing on the Kenai
  G        C     G                D
Them Dipterists frustrated at those gall flies
       Em                                 A7
Saying we're not close to done with these flies,
Em                                 D 
We're not close to done with these flies


(v2)

G                         Am
In this Great Land of we got a lotta
C                    Am
bebbs, barclay, and commuata
Em                               D
Each one with with its cecids makin' galls
       G      D          Em
Here is there one or are there six?
Am              C    
cecidomyiids eating Salix
Em                    Am         D
To start we'd need to find them all
Em                      Am
But how can these small flies be known?
Em                       Am
They're millimeters long, full grown
C           G          Am
With few characters to go on
C                    D
It has been quite a conundrum
        G       D        Em
There's morphology that we can use,
Am                          C
The damage and willows that they choose,
G              D             Em
Breeding and then there's molecules,
   C          D7     G
to know those cecid flies


(chorus)


(v3)
     G             Am
Well we tried to collect all kinds
   C                       Am
of willow rose galls that we could find
  Em                    D
all localities that we could round up
 G              D             Em
We pulled out larvae from their homes
       Am              C
On the table of our dining room
   Em          Am           D
into cryovials to be ground up 
   Em                     Am
We shipped them off, used homeschool funds
   Em                    Am
to read cytochrome oxidase one
C           G       Am
Waited to receive reads 
         C               D7   
Of those A's C's G's and T's
G         D                 Em
When they came after 'bout 30 days
        Am                  C
we used Clustal Omega and MrBayes
    G        D    Em
To scrutinize in fancy ways
    C           D7    G
The tree of our cecid flies


(chorus)


(v4)
   G          D              Em
We found our midges made two clear clades
Am                 C
Based on cytochrome oxidase
    Em                      D
The outgroup is another Rabdophaga
    G      D     Em
The first group ADA9342
 Am                  C
On Salix pulchra and fuscescens, too
  Em           C         D
It includes Old World rosaria
   Em                 Am
The next group seemed polyphagous
    Em                 Am
On a few more willows than we had guessed
 C     G    Am
But we did corroborate
 C               D
A finding that Dominique made
        G         D        Em   
Now we still must relate our OTUs
      Am             D
With latin names already used
 G          D          Em  
There is so much more work to do
 C            D7      G
to know those willow flies